PSEUDOMONAS CEPACIA AGAR
Pseudomonas Cepacia Agar is a selective agar medium used for the isolation of Burkholderia cepacia (formerly Pseudomonas cepacia) from clinical and environmental materials. Enzymatic Digest of Animal Tissue provides nitrogen, vitamins, and carbohydrates. Bile Extract and Crystal Violet are used to inhibit Gram-positive bacteria, while Polymyxin B and Ticarcillin suppress the growth of Gram-negative organisms. Magnesium Sulfate, Ammonium Sulfate, and Ferrous Ammonium Sulfate are used to promote the growth of Burkholderia cepacia. Dipotassium Phosphate and Monopotassium Phosphate serve as pH buffering agents. Sodium Pyruvate is utilized by B. cepacia, thus producing alkaline by-products and causing the Phenol Red indicator to turn pink to hot-pink in color. Agar is the gelifying agent.
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