EC Medium, otherwise known as EC Broth, is used to detect the presence of coliform bacteria in drinking water, wastewater, and foods. Developed by Hajna and Perryl, it was designed for coliform bacteria, specifically E. coli. A buffered Lactose broth, it contains Bile Salts to inhibit Gram-positive bacteria and spore-forming microorganisms while enhancing that of E. coli. Coliforms can be detected by growth at 37°C, while Escherichiacoli are detected by growth at 44.5°C. Dipotassium Phosphate and Monopotassium Phosphate act as buffering agents while Sodium Chloride maintains the osmotic balance of the medium.