Dilution: 25μL of 2X ACTaq™ Hot-Start Mastermix is required for each 50μL reaction.
Product Description: ACTaq™ Hot-Start Mastermix is complete “ready-to-go” heat –activated 2X reaction mix which only requires the consumer researcher to add water, template and primers, and then pre-heat to 94ºC for reaction activation. It is one of most convenient way to set up PCR reaction. Not only it comes with the advantage of Hot-Start Taq enzyme, it goes further by including all of the “ingredients” required for thermal cycling. Therefore, reduce the risk of contamination with ease and quick PCR setup, and ensuring greater reproducibility.
Hot-Start Activation Step: pre-incubate 2 minutes at 94ºC prior to thermal cycling.
Storage Condition: -20ºC. Stable for 12 months in constant freezer temperature.
Unit Definition: One unit of the amount of enzyme required to catalyze the incorporation of 1,000 nucleotides per minute at 65-72ºC.
Product Composition: 2.5 units of ACTaq™ HotStart DNA Polymerase (Antibody bound recombinant Taq DNA Polymerase); 20mM Tris-HCl (pH 9.0), 3.0mM MgCl2, 20mM KCl,16mM (NH4)2SO4, 0.1% Triton X-100, dNTP mix.
Quality Control: ACTaq™ Hot-Start DNA Polymerase is highly purified. Free of contaminating endonucleases, exonucleases, and nicking activities. The purity of the enzyme is evaluated by SDS-PAGE at >95% purity.